Review



si stat1  (Genecopoeia)


Bioz Verified Symbol Genecopoeia is a verified supplier
Bioz Manufacturer Symbol Genecopoeia manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Genecopoeia si stat1
    <t>STAT1</t> knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; <t>si-STAT1,</t> siRNA targeting STAT1; si-NC, negative control siRNA.
    Si Stat1, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/STAT1+Rabbit+mAb/pmc12930139-78-40-58
    Average 94 stars, based on 1 article reviews
    si stat1 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation"

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2026.13828

    STAT1 knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; si-STAT1, siRNA targeting STAT1; si-NC, negative control siRNA.
    Figure Legend Snippet: STAT1 knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; si-STAT1, siRNA targeting STAT1; si-NC, negative control siRNA.

    Techniques Used: Knockdown, Expressing, Western Blot, Cell Counting, Colony Assay, Small Interfering RNA, Negative Control

    STAT1 knockdown significantly promotes melanoma cell apoptosis and inhibits migration. (A and B) Apoptosis was assessed and quantified in A375 and RPMI-7951 cells following STAT1 knockdown using flow cytometry. (C) Transwell migration assays were conducted to evaluate cell migration following STAT1 knockdown. Magnification, ×200. (D) Quantification of migration capacity in A375 and RPMI-7951 cells following STAT1 knockdown. *P<0.05 vs. si-NC. STAT1, signal transducer and activator of transcription 1; si-STAT1, small interfering RNA targeting STAT1; si-NC, negative control small interfering RNA.
    Figure Legend Snippet: STAT1 knockdown significantly promotes melanoma cell apoptosis and inhibits migration. (A and B) Apoptosis was assessed and quantified in A375 and RPMI-7951 cells following STAT1 knockdown using flow cytometry. (C) Transwell migration assays were conducted to evaluate cell migration following STAT1 knockdown. Magnification, ×200. (D) Quantification of migration capacity in A375 and RPMI-7951 cells following STAT1 knockdown. *P<0.05 vs. si-NC. STAT1, signal transducer and activator of transcription 1; si-STAT1, small interfering RNA targeting STAT1; si-NC, negative control small interfering RNA.

    Techniques Used: Knockdown, Migration, Flow Cytometry, Small Interfering RNA, Negative Control

    STAT1 regulates TUBB4A expression at the transcription level. (A) STAT1 mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (B) TUBB4A mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (C) Specific fragments of the TUBB4A promoter region were cloned into the luciferase reporter plasmids upstream of the firefly luciferase gene. (D) Transcriptional activity of various TUBB4A promoter fragments was analyzed by luciferase reporter assay in 293T cells, with the −1,783 and −1,771 fragments exhibiting the highest activity. (E) STAT1 siRNA-mediated knockdown significantly reduced STAT1 mRNA levels in A375 cells. (F) STAT1 knockdown significantly reduced the luciferase activity of the −1,783 fragment of the TUBB4A promoter, but not the −1,771 fragment. (G) Chromatin immunoprecipitation assays were performed in A375 and RPMI-7951 cells targeting the −1,783 binding site in the TUBB4A promoter region. Quantitative PCR provided evidence of STAT1 binding to this region. Genomic DNA input was set to 100%. **P<0.01 vs. si-NC; ## P<0.01 vs. PGL3; && P<0.01 vs. IgG. STAT1, signal transducer and activator of transcription 1; siRNA, small interfering RNA; si-NC, negative control siRNA; si-STAT1, siRNA targeting STAT1; si-STAT1-1, siRNA targeting STAT1 sequence 1; si-STAT1-2, siRNA targeting STAT1 sequence 2; TUBB4A, tubulin β4A; PGL3, promoter-gluc luciferase 3; LUC, firefly luciferase gene.
    Figure Legend Snippet: STAT1 regulates TUBB4A expression at the transcription level. (A) STAT1 mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (B) TUBB4A mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (C) Specific fragments of the TUBB4A promoter region were cloned into the luciferase reporter plasmids upstream of the firefly luciferase gene. (D) Transcriptional activity of various TUBB4A promoter fragments was analyzed by luciferase reporter assay in 293T cells, with the −1,783 and −1,771 fragments exhibiting the highest activity. (E) STAT1 siRNA-mediated knockdown significantly reduced STAT1 mRNA levels in A375 cells. (F) STAT1 knockdown significantly reduced the luciferase activity of the −1,783 fragment of the TUBB4A promoter, but not the −1,771 fragment. (G) Chromatin immunoprecipitation assays were performed in A375 and RPMI-7951 cells targeting the −1,783 binding site in the TUBB4A promoter region. Quantitative PCR provided evidence of STAT1 binding to this region. Genomic DNA input was set to 100%. **P<0.01 vs. si-NC; ## P<0.01 vs. PGL3; && P<0.01 vs. IgG. STAT1, signal transducer and activator of transcription 1; siRNA, small interfering RNA; si-NC, negative control siRNA; si-STAT1, siRNA targeting STAT1; si-STAT1-1, siRNA targeting STAT1 sequence 1; si-STAT1-2, siRNA targeting STAT1 sequence 2; TUBB4A, tubulin β4A; PGL3, promoter-gluc luciferase 3; LUC, firefly luciferase gene.

    Techniques Used: Expressing, Knockdown, Transfection, Clone Assay, Luciferase, Activity Assay, Reporter Assay, Chromatin Immunoprecipitation, Binding Assay, Real-time Polymerase Chain Reaction, Small Interfering RNA, Negative Control, Sequencing

    TUBB4A overexpression mitigates the effects of STAT1 knockdown on cell viability and proliferation. TUBB4A mRNA levels were measured in (A) A375 and (B) RPMI-7951 cells following TUBB4A overexpression mediated by a lentiviral vector. (C) TUBB4A protein expression was analyzed after its overexpression. Combined STAT1 knockdown and TUBB4A overexpression transfections were performed, followed by a western blot analysis of TUBB4A protein levels in (D) A375 and (E) RPMI-7951 cells. Cell viability was assessed via Cell Counting Kit-8 assays following combined STAT1 knockdown and TUBB4A overexpression in (F) A375 and (G) RPMI-7951 cells. (H) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown and TUBB4A overexpression. (I) Quantification of colony formation assay results. **P<0.01 vs. Ov-NC; ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; STAT1, signal transducer and activator of transcription 1; si-NC, negative control small interfering RNA; si-STAT1, small interfering RNA targeting STAT1; Ov-NC, negative control lentiviral overexpression vector; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.
    Figure Legend Snippet: TUBB4A overexpression mitigates the effects of STAT1 knockdown on cell viability and proliferation. TUBB4A mRNA levels were measured in (A) A375 and (B) RPMI-7951 cells following TUBB4A overexpression mediated by a lentiviral vector. (C) TUBB4A protein expression was analyzed after its overexpression. Combined STAT1 knockdown and TUBB4A overexpression transfections were performed, followed by a western blot analysis of TUBB4A protein levels in (D) A375 and (E) RPMI-7951 cells. Cell viability was assessed via Cell Counting Kit-8 assays following combined STAT1 knockdown and TUBB4A overexpression in (F) A375 and (G) RPMI-7951 cells. (H) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown and TUBB4A overexpression. (I) Quantification of colony formation assay results. **P<0.01 vs. Ov-NC; ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; STAT1, signal transducer and activator of transcription 1; si-NC, negative control small interfering RNA; si-STAT1, small interfering RNA targeting STAT1; Ov-NC, negative control lentiviral overexpression vector; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.

    Techniques Used: Over Expression, Knockdown, Plasmid Preparation, Expressing, Transfection, Western Blot, Cell Counting, Colony Assay, Negative Control, Small Interfering RNA

    TUBB4A overexpression reverses the effects of STAT1 knockdown on apoptosis, migration and tumor growth. (A-D) Apoptosis and migration were evaluated in A375 and RPMI-7951 cells following STAT1 knockdown and TUBB4A overexpression. Magnification, ×200. (E) Representative images of isolated xenograft tumors in mice. Tumor volumes were measured in nude mice injected subcutaneously with 2×10 6 A375 cells that had been subject to STAT1 knockdown and TUBB4A overexpression. (F) Quantification of mouse tumor volumes showed that STAT1 knockdown significantly suppressed tumor growth, whereas TUBB4A overexpression reversed this inhibitory effect. ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; si-NC, negative control small interfering RNA; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.
    Figure Legend Snippet: TUBB4A overexpression reverses the effects of STAT1 knockdown on apoptosis, migration and tumor growth. (A-D) Apoptosis and migration were evaluated in A375 and RPMI-7951 cells following STAT1 knockdown and TUBB4A overexpression. Magnification, ×200. (E) Representative images of isolated xenograft tumors in mice. Tumor volumes were measured in nude mice injected subcutaneously with 2×10 6 A375 cells that had been subject to STAT1 knockdown and TUBB4A overexpression. (F) Quantification of mouse tumor volumes showed that STAT1 knockdown significantly suppressed tumor growth, whereas TUBB4A overexpression reversed this inhibitory effect. ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; si-NC, negative control small interfering RNA; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.

    Techniques Used: Over Expression, Knockdown, Migration, Isolation, Injection, Negative Control, Small Interfering RNA, Plasmid Preparation

    Related Articles

    Transfection:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: Absorbance was measured at 450 nm using a BioTek 800 TS Absorbance Reader (cat. no. ELx800; BioTek; Agilent Technologies, Inc.). .. Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.). .. After 10–14 days incubation, colonies were fixed with 4% paraformaldehyde (cat. no. J19943.K2; Thermo Fisher Scientific, Inc.) for 15 min, stained with 0.5% crystal violet solution (cat. no. C3886; MilliporeSigma; Merck KGaA) for 30 min and washed with phosphate-buffered saline (PBS; cat. no. 10010-023; Thermo Fisher Scientific, Inc.).

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: .. Melanoma cells (A375 and RPMI‐7951) were seeded into 6‐well plates (cat. no. 3516; Corning, Inc.) at a density of 500‐1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si‐NC (cat. no. A09010) or si‐STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov‐TUBB4A; pReceiver‐Lv105; GeneCopoeia, Inc.) or Ov‐NC (pReceiver‐Lv105 Empty Vector; GeneCopoeia, Inc.). .. After 10‐14 days incubation, colonies were fixed with 4% paraformaldehyde (cat. no. J19943.K2; Thermo Fisher Scientific, Inc.) for 15 min, stained with 0.5% crystal violet solution (cat. no. C3886; MilliporeSigma; Merck KGaA) for 30 min and washed with phosphate‐buffered saline (PBS; cat. no. 10010‐023; Thermo Fisher Scientific, Inc.).

    Over Expression:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: Absorbance was measured at 450 nm using a BioTek 800 TS Absorbance Reader (cat. no. ELx800; BioTek; Agilent Technologies, Inc.). .. Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.). .. After 10–14 days incubation, colonies were fixed with 4% paraformaldehyde (cat. no. J19943.K2; Thermo Fisher Scientific, Inc.) for 15 min, stained with 0.5% crystal violet solution (cat. no. C3886; MilliporeSigma; Merck KGaA) for 30 min and washed with phosphate-buffered saline (PBS; cat. no. 10010-023; Thermo Fisher Scientific, Inc.).

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: .. Melanoma cells (A375 and RPMI‐7951) were seeded into 6‐well plates (cat. no. 3516; Corning, Inc.) at a density of 500‐1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si‐NC (cat. no. A09010) or si‐STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov‐TUBB4A; pReceiver‐Lv105; GeneCopoeia, Inc.) or Ov‐NC (pReceiver‐Lv105 Empty Vector; GeneCopoeia, Inc.). .. After 10‐14 days incubation, colonies were fixed with 4% paraformaldehyde (cat. no. J19943.K2; Thermo Fisher Scientific, Inc.) for 15 min, stained with 0.5% crystal violet solution (cat. no. C3886; MilliporeSigma; Merck KGaA) for 30 min and washed with phosphate‐buffered saline (PBS; cat. no. 10010‐023; Thermo Fisher Scientific, Inc.).



    Similar Products

    si stat1  (Genecopoeia)
    94
    Genecopoeia si stat1
    <t>STAT1</t> knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; <t>si-STAT1,</t> siRNA targeting STAT1; si-NC, negative control siRNA.
    Si Stat1, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/STAT1+Rabbit+mAb/pmc12930139-78-40-58
    Average 94 stars, based on 1 article reviews
    si stat1 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    sirna sets of si-ifnar1, si-ifnar2, si-jak1, si-stat1 and si-tyk2  (Thermo Fisher)
    90
    Thermo Fisher sirna sets of si-ifnar1, si-ifnar2, si-jak1, si-stat1 and si-tyk2
    <t>STAT1</t> knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; <t>si-STAT1,</t> siRNA targeting STAT1; si-NC, negative control siRNA.
    Sirna Sets Of Si Ifnar1, Si Ifnar2, Si Jak1, Si Stat1 And Si Tyk2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/anti+stat1+antibody+aho0832/pmc11754596-369-9-16
    Average 90 stars, based on 1 article reviews
    sirna sets of si-ifnar1, si-ifnar2, si-jak1, si-stat1 and si-tyk2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    si-stat1  (Thermo Fisher)
    90
    Thermo Fisher si-stat1
    A Interaction of p53 and <t>STAT1</t> in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.
    Si Stat1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/pmc11754596-369-7-16
    Average 90 stars, based on 1 article reviews
    si-stat1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    small interfering rna si-stat1  (Ribobio co)
    90
    Ribobio co small interfering rna si-stat1
    A Interaction of p53 and <t>STAT1</t> in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.
    Small Interfering Rna Si Stat1, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/stat1+sirna/pm39237779-59-17-27
    Average 90 stars, based on 1 article reviews
    small interfering rna si-stat1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    si-stat1  (Nova Biomedical)
    90
    Nova Biomedical si-stat1
    A Interaction of p53 and <t>STAT1</t> in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.
    Si Stat1, supplied by Nova Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/si+stat1/pmc10069069-69-5-19
    Average 90 stars, based on 1 article reviews
    si-stat1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    sirna si- stat1  (Ribobio co)
    90
    Ribobio co sirna si- stat1
    A Interaction of p53 and <t>STAT1</t> in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.
    Sirna Si Stat1, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/stat1+sirna/10__1096_slash_fj__202201582r-32-0-19
    Average 90 stars, based on 1 article reviews
    sirna si- stat1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    non-silencing control plasmids (si-nc and ctr-stat1)  (Ribobio co)
    90
    Ribobio co non-silencing control plasmids (si-nc and ctr-stat1)
    A Interaction of p53 and <t>STAT1</t> in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.
    Non Silencing Control Plasmids (Si Nc And Ctr Stat1), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/stat1+sirna/pm37187897-100-7-17
    Average 90 stars, based on 1 article reviews
    non-silencing control plasmids (si-nc and ctr-stat1) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    stat1 sirna (si-stat1)  (Ribobio co)
    90
    Ribobio co stat1 sirna (si-stat1)
    A Interaction of p53 and <t>STAT1</t> in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.
    Stat1 Sirna (Si Stat1), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si+stat1/stat1+sirna/pm37187897-100-0-17
    Average 90 stars, based on 1 article reviews
    stat1 sirna (si-stat1) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    STAT1 knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; si-STAT1, siRNA targeting STAT1; si-NC, negative control siRNA.

    Journal: Molecular Medicine Reports

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

    doi: 10.3892/mmr.2026.13828

    Figure Lengend Snippet: STAT1 knockdown significantly reduces melanoma cell viability and proliferation. STAT1 mRNA expression was analyzed in (A) A375 and (B) RPMI-7951 melanoma cells post-STAT1 knockdown using siRNA. (C) Western blotting was used to assess STAT1 protein levels in melanoma cell lines following siRNA-mediated knockdown. Cell viability was evaluated after STAT1 knockdown in (D) A375 and (E) RPMI-7951 cells using Cell Counting Kit-8 assays. (F) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown. (G) Quantification of colony formation assay results. **P<0.01 vs. si-NC group. siRNA, small interfering RNA; STAT1, signal transducer and activator of transcription 1; si-STAT1, siRNA targeting STAT1; si-NC, negative control siRNA.

    Article Snippet: Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.).

    Techniques: Knockdown, Expressing, Western Blot, Cell Counting, Colony Assay, Small Interfering RNA, Negative Control

    STAT1 knockdown significantly promotes melanoma cell apoptosis and inhibits migration. (A and B) Apoptosis was assessed and quantified in A375 and RPMI-7951 cells following STAT1 knockdown using flow cytometry. (C) Transwell migration assays were conducted to evaluate cell migration following STAT1 knockdown. Magnification, ×200. (D) Quantification of migration capacity in A375 and RPMI-7951 cells following STAT1 knockdown. *P<0.05 vs. si-NC. STAT1, signal transducer and activator of transcription 1; si-STAT1, small interfering RNA targeting STAT1; si-NC, negative control small interfering RNA.

    Journal: Molecular Medicine Reports

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

    doi: 10.3892/mmr.2026.13828

    Figure Lengend Snippet: STAT1 knockdown significantly promotes melanoma cell apoptosis and inhibits migration. (A and B) Apoptosis was assessed and quantified in A375 and RPMI-7951 cells following STAT1 knockdown using flow cytometry. (C) Transwell migration assays were conducted to evaluate cell migration following STAT1 knockdown. Magnification, ×200. (D) Quantification of migration capacity in A375 and RPMI-7951 cells following STAT1 knockdown. *P<0.05 vs. si-NC. STAT1, signal transducer and activator of transcription 1; si-STAT1, small interfering RNA targeting STAT1; si-NC, negative control small interfering RNA.

    Article Snippet: Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.).

    Techniques: Knockdown, Migration, Flow Cytometry, Small Interfering RNA, Negative Control

    STAT1 regulates TUBB4A expression at the transcription level. (A) STAT1 mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (B) TUBB4A mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (C) Specific fragments of the TUBB4A promoter region were cloned into the luciferase reporter plasmids upstream of the firefly luciferase gene. (D) Transcriptional activity of various TUBB4A promoter fragments was analyzed by luciferase reporter assay in 293T cells, with the −1,783 and −1,771 fragments exhibiting the highest activity. (E) STAT1 siRNA-mediated knockdown significantly reduced STAT1 mRNA levels in A375 cells. (F) STAT1 knockdown significantly reduced the luciferase activity of the −1,783 fragment of the TUBB4A promoter, but not the −1,771 fragment. (G) Chromatin immunoprecipitation assays were performed in A375 and RPMI-7951 cells targeting the −1,783 binding site in the TUBB4A promoter region. Quantitative PCR provided evidence of STAT1 binding to this region. Genomic DNA input was set to 100%. **P<0.01 vs. si-NC; ## P<0.01 vs. PGL3; && P<0.01 vs. IgG. STAT1, signal transducer and activator of transcription 1; siRNA, small interfering RNA; si-NC, negative control siRNA; si-STAT1, siRNA targeting STAT1; si-STAT1-1, siRNA targeting STAT1 sequence 1; si-STAT1-2, siRNA targeting STAT1 sequence 2; TUBB4A, tubulin β4A; PGL3, promoter-gluc luciferase 3; LUC, firefly luciferase gene.

    Journal: Molecular Medicine Reports

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

    doi: 10.3892/mmr.2026.13828

    Figure Lengend Snippet: STAT1 regulates TUBB4A expression at the transcription level. (A) STAT1 mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (B) TUBB4A mRNA levels were measured in A375 and RPMI-7951 cells after STAT1 knockdown via transfection with different siRNA sequences. (C) Specific fragments of the TUBB4A promoter region were cloned into the luciferase reporter plasmids upstream of the firefly luciferase gene. (D) Transcriptional activity of various TUBB4A promoter fragments was analyzed by luciferase reporter assay in 293T cells, with the −1,783 and −1,771 fragments exhibiting the highest activity. (E) STAT1 siRNA-mediated knockdown significantly reduced STAT1 mRNA levels in A375 cells. (F) STAT1 knockdown significantly reduced the luciferase activity of the −1,783 fragment of the TUBB4A promoter, but not the −1,771 fragment. (G) Chromatin immunoprecipitation assays were performed in A375 and RPMI-7951 cells targeting the −1,783 binding site in the TUBB4A promoter region. Quantitative PCR provided evidence of STAT1 binding to this region. Genomic DNA input was set to 100%. **P<0.01 vs. si-NC; ## P<0.01 vs. PGL3; && P<0.01 vs. IgG. STAT1, signal transducer and activator of transcription 1; siRNA, small interfering RNA; si-NC, negative control siRNA; si-STAT1, siRNA targeting STAT1; si-STAT1-1, siRNA targeting STAT1 sequence 1; si-STAT1-2, siRNA targeting STAT1 sequence 2; TUBB4A, tubulin β4A; PGL3, promoter-gluc luciferase 3; LUC, firefly luciferase gene.

    Article Snippet: Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.).

    Techniques: Expressing, Knockdown, Transfection, Clone Assay, Luciferase, Activity Assay, Reporter Assay, Chromatin Immunoprecipitation, Binding Assay, Real-time Polymerase Chain Reaction, Small Interfering RNA, Negative Control, Sequencing

    TUBB4A overexpression mitigates the effects of STAT1 knockdown on cell viability and proliferation. TUBB4A mRNA levels were measured in (A) A375 and (B) RPMI-7951 cells following TUBB4A overexpression mediated by a lentiviral vector. (C) TUBB4A protein expression was analyzed after its overexpression. Combined STAT1 knockdown and TUBB4A overexpression transfections were performed, followed by a western blot analysis of TUBB4A protein levels in (D) A375 and (E) RPMI-7951 cells. Cell viability was assessed via Cell Counting Kit-8 assays following combined STAT1 knockdown and TUBB4A overexpression in (F) A375 and (G) RPMI-7951 cells. (H) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown and TUBB4A overexpression. (I) Quantification of colony formation assay results. **P<0.01 vs. Ov-NC; ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; STAT1, signal transducer and activator of transcription 1; si-NC, negative control small interfering RNA; si-STAT1, small interfering RNA targeting STAT1; Ov-NC, negative control lentiviral overexpression vector; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.

    Journal: Molecular Medicine Reports

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

    doi: 10.3892/mmr.2026.13828

    Figure Lengend Snippet: TUBB4A overexpression mitigates the effects of STAT1 knockdown on cell viability and proliferation. TUBB4A mRNA levels were measured in (A) A375 and (B) RPMI-7951 cells following TUBB4A overexpression mediated by a lentiviral vector. (C) TUBB4A protein expression was analyzed after its overexpression. Combined STAT1 knockdown and TUBB4A overexpression transfections were performed, followed by a western blot analysis of TUBB4A protein levels in (D) A375 and (E) RPMI-7951 cells. Cell viability was assessed via Cell Counting Kit-8 assays following combined STAT1 knockdown and TUBB4A overexpression in (F) A375 and (G) RPMI-7951 cells. (H) Colony formation assays were performed to assess the proliferative capacity of cells after STAT1 knockdown and TUBB4A overexpression. (I) Quantification of colony formation assay results. **P<0.01 vs. Ov-NC; ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; STAT1, signal transducer and activator of transcription 1; si-NC, negative control small interfering RNA; si-STAT1, small interfering RNA targeting STAT1; Ov-NC, negative control lentiviral overexpression vector; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.

    Article Snippet: Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.).

    Techniques: Over Expression, Knockdown, Plasmid Preparation, Expressing, Transfection, Western Blot, Cell Counting, Colony Assay, Negative Control, Small Interfering RNA

    TUBB4A overexpression reverses the effects of STAT1 knockdown on apoptosis, migration and tumor growth. (A-D) Apoptosis and migration were evaluated in A375 and RPMI-7951 cells following STAT1 knockdown and TUBB4A overexpression. Magnification, ×200. (E) Representative images of isolated xenograft tumors in mice. Tumor volumes were measured in nude mice injected subcutaneously with 2×10 6 A375 cells that had been subject to STAT1 knockdown and TUBB4A overexpression. (F) Quantification of mouse tumor volumes showed that STAT1 knockdown significantly suppressed tumor growth, whereas TUBB4A overexpression reversed this inhibitory effect. ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; si-NC, negative control small interfering RNA; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.

    Journal: Molecular Medicine Reports

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

    doi: 10.3892/mmr.2026.13828

    Figure Lengend Snippet: TUBB4A overexpression reverses the effects of STAT1 knockdown on apoptosis, migration and tumor growth. (A-D) Apoptosis and migration were evaluated in A375 and RPMI-7951 cells following STAT1 knockdown and TUBB4A overexpression. Magnification, ×200. (E) Representative images of isolated xenograft tumors in mice. Tumor volumes were measured in nude mice injected subcutaneously with 2×10 6 A375 cells that had been subject to STAT1 knockdown and TUBB4A overexpression. (F) Quantification of mouse tumor volumes showed that STAT1 knockdown significantly suppressed tumor growth, whereas TUBB4A overexpression reversed this inhibitory effect. ## P<0.01 vs. si-STAT1. TUBB4A, tubulin β4A; si-NC, negative control small interfering RNA; Ov-TUBB4A, lentiviral vector for TUBB4A overexpression.

    Article Snippet: Melanoma cells (A375 and RPMI-7951) were seeded into 6-well plates (cat. no. 3516; Corning, Inc.) at a density of 500–1,000 cells per well and allowed to adhere for 24 h. Cells were then transfected with si-NC (cat. no. A09010) or si-STAT1 (cat. no. A09009; GenePharma), as well as transfected using a lentiviral vector for TUBB4A overexpression (Ov-TUBB4A; pReceiver-Lv105; GeneCopoeia, Inc.) or Ov-NC (pReceiver-Lv105 Empty Vector; GeneCopoeia, Inc.).

    Techniques: Over Expression, Knockdown, Migration, Isolation, Injection, Negative Control, Small Interfering RNA, Plasmid Preparation

    A Interaction of p53 and STAT1 in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.

    Journal: Cell Death & Disease

    Article Title: Tp53 determines the spatial dynamics of M1/M2 tumor-associated macrophages and M1-driven tumoricidal effects

    doi: 10.1038/s41419-025-07346-0

    Figure Lengend Snippet: A Interaction of p53 and STAT1 in M1 CM. Immunoprecipitation of p53 using anti-p53 antibody (FL-393) and immunoblot using anti-STAT1 and anti-p53 (DO-1) antibodies. Cell lysate was prepared from A549 cells treated with M0 or M1 CM for 3 days. B Effect of STAT1 silencing on M1-induced apoptosis in A549 cells. NC: siRNA negative control. Data are represented as mean ± SD; n = 4. P -value determined by two-way ANOVA with Tukey’s post hoc test. See Supplementary Fig. for gating strategy of apoptosis assay. C Effect of STAT1 silencing on p53 protein stability in A549 cells. The M1-culture cells were treated with 100 μg/ml cycloheximide and harvested as indicated duration. NC siRNA negative control. D The interaction of p53 and MDM2 in STAT1 knockdown cells. si-STAT1 was delivered into H1299 cells, followed by transfection with p53 and MDM2. After culture in M1 CM for 30 h, cells were treated with 30 μM MG132 for 4 h prior to harvest. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. E Ubiquitination of p53 in H1299 cells overexpressing STAT1. Cells transfected with Flag-wtp53, STAT1, and Ub were cultured in M0 or M1 CM for 30 h, followed by treatment with 30 μM MG132 for 4 h before harvesting. Immunoprecipitation was performed with an anti-p53 antibody (GTX102965) and analyzed by immunoblot. F The interaction of p53 and phosphorylated STAT1 in M0 and M1 CM treated A549 cells. Cells were culture in M0 and M1 CM for 72 h and then harvested. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The phosphorylation of STAT1 was assayed by anti-pY701-STAT1 (58D6). G Interaction between p53 and STAT1 in A549 cells treated with M1 CM and neutralizing antibodies against IFN-γ and IFN-β. Cells were culture M1 CM with IgG control or the combination of IFN-γ and IFN-β neutralizing antibodies (nAb) for 48 h. Prior to harvest, cells were treated with 30 μM MG132 for 4 h. Lysates were immunoprecipitated with anti-p53 (DO-1) antibody and analyzed by immunoblot. The combination treatment included 1 μg/ml each of IFN-β and IFN-γ nAbs and 2 μg/ml IgG as the negative control. H Interaction of p53 with either STAT1 or Y701F mutant. HEK293 cells co-transfected with Flag-p53 and either HA-STAT1 or HA-STAT1-Y701F vectors were immunoprecipitated with anti-Flag antibody and assayed by immunoblot with anti-Flag and anti-HA antibodies. I Effect of a dominant negative STAT1-Y701F mutant on the p53 protein stability. H1299 cells, expressing either STAT1 or STAT1-Y701F, were treated with M1 CM and then with 100 μg/ml cycloheximide, and samples were collected at indicated durations. Data are representative of at least two independent experiments.

    Article Snippet: The siRNA sets of si-IFNAR1, si-IFNAR2, si-JAK1, si-STAT1 and si-TYK2 were purchased from Silencer® Select siRNA (Thermo Fisher Scientific).

    Techniques: Immunoprecipitation, Western Blot, Negative Control, Apoptosis Assay, Knockdown, Transfection, Cell Culture, Control, Mutagenesis, Dominant Negative Mutation, Expressing